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the lung should prevent the bronchoconstriction associated with asthma. Considerable work based on this concept has been occasioned by the observation that quaternary salts of atropine (93), which should not be absorbed systemically, do retain the anticholinergic activity of the parent base. One such salt, ipratropium bromide (92), has undergone considerable clinical investigation as an antiasthmatic agent administered by insufflation (j^e*, topical application to the bronchioles). The fact that the stereochemistry of this agent is the opposite from that which would be obtained by direct alkylation with isopropyl bromide requires that a somewhat longer sequence be employed for its synthesis. Preparation of the key tropine jS6^ is achieved by any one of several variations on the method first developed by Robinson, which involves reaction of a primary amine with dihydroxyacetone and glyoxal. Reduction of the carbonyl group in the product (86) followed by acylation affords the aminoester (88). Transesterification with ester aldehyde 89 C ( H) HC 37 2 I (89) (90) R - CO H (91) R = C O HI , . _ CCH,l2CH>.+^CI.3 r (92) (93)

Creating a hierarchical skeleton that is used to control the animation of the mesh skin that is draped over it is quite easy when you create a biped. The skeleton can be set to be invisible in the final render and exists only to make the process of animating easier. Although Max includes a robust set of tools that can be used to create a skeleton of bones, the Biped feature automates this entire process using prebuilt skeletons.

When glass shatters, it is very chaotic, sending pieces in every direction For this tutorial, we shatter a glass mirror on a wall The wall keeps the pieces from flying off, and most pieces fall straight to the floor To shatter glass, follow these steps: 1 Open the Shattering glassmax file from the Chap 42 directory on the DVD This file includes a simple mirror created from patch grid objects The file also includes a simple sphere that is animated striking the mirror 2 Select the Create Particles PArray menu command Then drag in the Front viewport to create the PArray icon In the Basic Parameters rollout, click the Pick Object button and select the first patch object In the Viewport Display section, select the Mesh option In the Particle Generation rollout, set the Speed and Divergence to 0.

Crucially for the study of cell death, only the amoeba cells in the sphere atop the stalk go on to make spores These will be taken by the wind to new soil sites where new colonies of amoebas can grow But what about the cells in the supportive stalk Those die They are sacrifices for the cells that make the spores The stalk cells give their lives to lift the others above the substrate so the spores of those others are propelled into air currents144 Here is yet another example of cell suicide in single-celled creatures that is related to a social situation Recently, lab experiments using the amoeba slime mold have revealed that cheats can exist145 Researchers took cells from the same locale in nature and found genetic variants.

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These variants could be mixed in randomly paired populations and the resulting mixed colony induced to go into first the slug phase and then the phase of stalk and spore-forming ball The key discovery was that some variants were able to preferentially put their cells into the ball and not the stalk These forceful cells were not willing to die as part of the stalk So in these experimental chimeras, one genome would produce more spores than the other genome, whose cells primarily went into the supporting stalk This is a case of mixed genomes with potential conflicts of interest Do the chimeras occur in nature That is not known Presumably not Researchers suspect there are one or more isolating mechanisms that keep the variants apart, thwarting the possibility of cheating in the suicide game.

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In general, a population that can undergo selective cell death of some of its cells is most likely to be evolutionarily stable when the cells that die are in some sense helping their same genetic selves..

Another method of minimizing the nancial cost of bad debts is to insure invoiced sales against the debt going bad. Bad debt insurance can be quite expensive, but could be used selectively for larger or more risky sales, such as those to foreign customers, where a comprehensive package of nancial and other trading assistance should be sought.